Parameters and methods
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A summary of method descriptions and material used during the PREFIL cruises is given here below.
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Meteo
| P2 | P3 | P4 | P5 | P6 | P7 | P8 | P9 | P10 | ||||
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-Dry air temperature -Wet air temperature -Wind direction and velocity |
Board Met. Station | All stations |
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R. Le Borgne |
Currentology
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Zonal current component |
GEK (ORSTOM made) | Continuous |
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R. Le Borgne | ||||
| Zonal current component | Aandera current profiler | Drifting buoy |
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R. Le Borgne |
| P2 | P3 | P4 | P5 | P6 | P7 | P8 | P9 | P10 | ||||
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-Water temperature -Salinity -Density |
Bisset-Berman CTD probe, downward measurements | all stations |
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R. Le Borgne |
| -Light energy/day | LI-COR quantameter | long stations |
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R. Le Borgne |
| -Z/surface light intensity ratio | LI-COR quantameter | long stations |
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R. Le Borgne |
| -Fluorometry+ DCMU /in vivo fluorometry | long stations |
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R. Le Borgne |
| P2 | P3 | P4 | P5 | P6 | P7 | P8 | P9 | P10 | ||||
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Nutrients (NO3, NO2, NH4 ,PO4 ) |
Technicon auto-analyzer (Manuel "Barrier/ORSTOM): methods in Grasshof et al.(1983) for NH4 and Strickland & Parsons (1972) for other nutrients | all stations |
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M. Rodier |
| Particulate matter |
-C,N: 1 to 1.5 litres on Ag Selas Flotronics filters (0.8 µm) from P2 to P8 and Whatman GF/F for P9 and P10 / HP185 CHN analyzer -P: 1.5 litres on Whatman GF/F / Method in Menzel & Corwin (1965) |
long stations/ sampling with 30l Niskin bottle |
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M. Rodier |
| Dissolved oxygen | Winkler's method (Strickland & Parsons, 1972) | all stations |
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M. Rodier |
| % oxygen saturation | Weiss, 1970 | all stations |
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M. Rodier |
| Apparent oxygen utilisation | Weiss, 1970 | all stations |
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M. Rodier |
| P2 | P3 | P4 | P5 | P6 | P7 | P8 | P9 | P10 | ||||
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Aceton extracted chlorophyllian pigments |
Filtration on Whatman GF/F / Fluorometry on aceton extracts (after acidification for pheopigments) according to Holm-Hansen et al. (1965) | all stations |
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A. Le Bouteiller | |
| "In-vivo" chlorophyll | "in-vivo"fluorescence, converted into chlorophyll a using calibration values obtained for 3 to 4 depths, on aceton extracts. | all stations |
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A. Le Bouteiller |
| P2 | P3 | P4 | P5 | P6 | P7 | P8 | P9 | P10 | ||||
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14C uptake |
liquid scintillation counting (Instagel and Aquasol 2)/ Primary Prod. calculations used total CO2 = 90 mg/l | long stations/ 30 l Nikin bottle sampling |
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A. Le Bouteiller |
| P2 | P3 | P4 | P5 | P6 | P7 | P8 | P9 | P10 | ||||
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Numbers of copepods |
Dissecting microscope/ 27l sieved on 200µm | long stations/30 l Niskin bottle |
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R. Le Borgne | |
| Biomass and elemental composition |
-oven (60°C) and deep-freezing on board. Weighing in land lab. Furnace (550°C, 1.5h) for AFDW -%C, N and P:diluted plankton grinds dried on board. Weighing in land lab. C and N as for particulate matter. P by method of Menzel & Corwin (1965) Methods in Le Borgne (1975) |
some stations/vertical WP-2 nets |
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R. Le Borgne | |
| Microzooplankton (50-200 µm) and mesozooplankton (>200 µm) concentration. | Particulate phosphorus of sieved samples (Menzel & Corwin, 1965) | 30 l Niskin bottle |
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R. Le Borgne | ||
| Respiration and excretion rates | Incubations. Analytical methods described above. For NT and PT, Armstrong & Tibbitts (1968) | some stations/vertical WP-2 nets |
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R. Le Borgne | |
| Taxonomic composition (micro- and mesozooplankton) | Counts and weighings on formalin fixed samples | all stations/ vertical WP-2 nets |
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R. Le Borgne | |
| Macrozooplankton and micronekton taxonomic composition | Counts and weighings (60°C, 48h) on formalin fixed samples | long stations /oblique OMORI net |
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R. Le Borgne |
Zooplankton sampling procedures:
microzooplankton: vertical 50 µm mesh net, with a 39 cm diameter (Ph in tables) from P3 to P7 / double net (PhBl in tables). Plancton was sieved on a 200 µm metal grid.
mesozooplankton: vertical triple WP-2 (UNESCO, 1968) nets with a 200 µm mesh aperture. Samples sieved on a 5 mm metal grid.
macrozooplankton and micronekton: oblique OMORI (1965) net, diameter = 160 cm, mesh aperture = 2 mm.
References: